Dh7 serum 2 different ones
HSCs can presumptively characterize and isolated with the help of an anticipated set of clusters of differentiation antigens and cell surface marker present on their cell membrane as predicted and proven by Irving L. Somatic cells can also become the source of HSCs by exploiting Induced pluripotent stem cells (IPSCs) ( 3). Moreover, the umbilical cord blood (UCB) and placenta, generally discarded after childbirth, are rich sources of HSCs ( 2).
Also, it is feasible to obtain HSCs and various progenitors of the blood cells from fetal as well as embryonic tissues. Apart from bone marrow, which is their reservoir, HSCs can also be isolated from granulocyte-colony stimulating factor (G-CSF) mobilized peripheral blood. ( 1).Īmong all the stem cells, hematopoietic stem cells (HSCs), responsible for hematopoiesis in the adult body are the utmost explored and researched one, owing to their enormous potential as well as therapeutic applications. Based on the source of isolation, stem cells can be embryonic, fetal, or adult which can be cord blood, placental, bone marrow (BM), dental, adipose tissue, skin, etc. Being unspecialized, these cells retain self-renewal capacity, hence regenerate their pool by cell division symmetric or asymmetric which further leads to differentiation into diverse cell types. Stem cells, an emerging tool for regenerative medicine, are disparate from other cell types of our body owing to exclusive properties they embrace. Here in this review, we are assembling various types of hematopoietic stem cell self-renewal, expansion and differentiation media along with supplements and culture conditions, either developed and used by various scientists or are available commercially. Media commercialization for precise stem cell types, culturing and differentiation is a cost-effective developing field. Maintenance of hematopoietic stem and progenitor cell lines along with the advancement of research work generate an inexorable demand for production and commercialization of specialized stem cell culture media, with or without serum along with specific growth factors and supplements. The precise equilibrium between self-renewal and differentiation of stem cells sustained by exclusive medium along with special growth or differentiation factors is accountable for generating diverse cell lineages.
Since these cells remain in senescence for a prolonged period before isolation, selection of appropriate growth medium along with supplements and culture conditions are crucial to initiate their cell division and to designate their destiny.
Enhanced usage of hematopoietic stem cells in research and therapeutics intensified the requirement of expansion and differentiation of hematopoietic stem cells under in vitro conditions. Although these stem cells are the most investigated and exploited until now, further research is still essential to comprehend their nature, fate, and potential. Owing to differentiation and self-renewal capacity, hematopoietic stem cells clasp potentiality to engender all blood cell types, leading to their immense competence to play a diverse role in therapeutic applications.